human liver hepatocellular carcinoma cell line hepg2  (ATCC)

Journal: Discover Oncology

Article Title: Isosilybin B: a potential novel therapeutic agent with hepatoprotective, anticancer and antifibrotic properties

doi: 10.1007/s12672-025-03380-8

Figure Lengend Snippet: Effect of IB/SB/SM on cytotoxicity and cell cycle arrest of liver cells. A : Chemical structure of IB and SB (silybin A and silybin B in ratio 1:1). Cytotoxicity in Hepa1-6 ( B ), HepG2 ( C ) or AML12 ( D ) liver cells after 24 h treatment with IB/SB/SM. Changes in the cell cycle in Hepa1-6 ( E ), HepG2 ( F ), or AML12 ( G ) liver cells after 24 h treatment with 31.3 µg/mL of IB/SB/SM. H : Cell distribution of diploid cells in the phases of the cell cycle shown for IB (31.3 µg/mL). The data are given as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Human liver hepatocellular carcinoma cell line HepG2 (RRID: CVCL_0027, ATCC, USA) was cultivated in RPMI medium supplemented with 10% FBS and 1% penicillin-streptomycin.

Techniques:

Journal: Research in Pharmaceutical Sciences

Article Title: Synthesis and in vitro evaluation of self-assembling biocompatible heparin-based targeting polymeric micelles for delivery of doxorubicin to leukemic cells

doi: 10.4103/RPS.RPS_197_24

Figure Lengend Snippet: Fluorescent and visible light microscopy images of K562 (rows 1 and 2) and HepG2 (rows 3 and 4) cells incubated with culture medium, free DOX, DOX/HEP-DEX, and DOX/FA-HEP-DEX micelles after 4 h incubation. DOX, Doxorubicin; FA, folic acid; HEP, heparin; DEX, dexamethasone.

Article Snippet: Human erythroleukemic (K562) and human liver hepatocellular carcinoma (HepG2) cell line s were supplied by the Pasteur Institute of Iran (Iran, Tehran).

Techniques: Light Microscopy, Incubation

Journal: Research in Pharmaceutical Sciences

Article Title: Synthesis and in vitro evaluation of self-assembling biocompatible heparin-based targeting polymeric micelles for delivery of doxorubicin to leukemic cells

doi: 10.4103/RPS.RPS_197_24

Figure Lengend Snippet: The cellular uptake percentage of free DOX, DOX/HEP-DEX, and DOX/FA-HEP-DEX micelles after 4 h incubation in K562 and HepG2 cells. DOX, Doxorubicin; FA, folic acid; HEP, heparin; DEX, dexamethasone.

Article Snippet: Human erythroleukemic (K562) and human liver hepatocellular carcinoma (HepG2) cell line s were supplied by the Pasteur Institute of Iran (Iran, Tehran).

Techniques: Incubation

Journal: Research in Pharmaceutical Sciences

Article Title: Synthesis and in vitro evaluation of self-assembling biocompatible heparin-based targeting polymeric micelles for delivery of doxorubicin to leukemic cells

doi: 10.4103/RPS.RPS_197_24

Figure Lengend Snippet: In vitro cytotoxicity of free DOX, DOX-loaded micelles, and blank micelles evaluated against (A) K562 cells after 48 h incubation, (B) K562 cells after 72 h incubation, (C) HepG2 cells after 48 h incubation, and (D) HepG2 cells after 72 h incubation. Data are plotted as the mean ± SD, n = 3. DOX, Doxorubicin; FA, folic acid; HEP, heparin; DEX, dexamethasone.

Article Snippet: Human erythroleukemic (K562) and human liver hepatocellular carcinoma (HepG2) cell line s were supplied by the Pasteur Institute of Iran (Iran, Tehran).

Techniques: In Vitro, Incubation

Journal: Research in Pharmaceutical Sciences

Article Title: Synthesis and in vitro evaluation of self-assembling biocompatible heparin-based targeting polymeric micelles for delivery of doxorubicin to leukemic cells

doi: 10.4103/RPS.RPS_197_24

Figure Lengend Snippet: IC 50 values of DOX from free DOX, DOX/FA-HEP-DEX and DOX/HEP-DEX micelles after 48 h and 72 h exposure to K562 and HepG2 cells. Data represent mean ± SD, n = 3. ** P < 0.01 and *** P < 0.001 represent significant differences in comparison with free DOX; ## P < 0.01 versus DOX/HEP-DEX.

Article Snippet: Human erythroleukemic (K562) and human liver hepatocellular carcinoma (HepG2) cell line s were supplied by the Pasteur Institute of Iran (Iran, Tehran).

Techniques: Comparison

Journal: BMC complementary medicine and therapies

Article Title: Effects of essential oil of Origanum onites and its major component carvacrol on the expression of toxicity pathway genes in HepG2 cells.

doi: 10.1186/s12906-024-04571-6

Figure Lengend Snippet: Fig. 2 Inhibition effects of DNA synthesis from the EOOO. (0.02–0.2 µg/mL) (A) and CV (7.5–105 µg/mL) (B) on the growth of HepG2 cells after 48 h. The EOOO and CV induced inhibition of DNA syntheses were in a concentration-dependent manner by the method BrdU incorporation assay. All values are expressed as mean ± SD at least three separate experiments performed in quadruplicate. Differences were considered significant compared to the control group from *p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001

Article Snippet: A human liver hepatocellular carcinoma cell line HepG2 purchased from DSMZ (Braunschweig, Germany) was cultured in DMEM (Dulbecco Modified Eagle Medium) (Sigma) supplemented with 10% FBS (fetal bovine serum) (PAA Lab. GmbH, Les Mureaux, France), penicillin/ streptomycin at 100 units/ml and 2 mM L-glutamine as adherent monolayers.

Techniques: Inhibition, DNA Synthesis, Concentration Assay, BrdU Incorporation Assay, Control

Journal: BMC complementary medicine and therapies

Article Title: Effects of essential oil of Origanum onites and its major component carvacrol on the expression of toxicity pathway genes in HepG2 cells.

doi: 10.1186/s12906-024-04571-6

Figure Lengend Snippet: Fig. 3 Effects of the EOOO (A) (0.08 and 0.09 µg/mL) and (B) CV (45 and 75 µg/mL) on viability of HepG2 cells incubated for 24, 48, 72 and 96 h. Each value is the mean ± S.D. of three separate experiments performed in quadruplicate. Differences were considered significant compared to the control group from *p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001

Article Snippet: A human liver hepatocellular carcinoma cell line HepG2 purchased from DSMZ (Braunschweig, Germany) was cultured in DMEM (Dulbecco Modified Eagle Medium) (Sigma) supplemented with 10% FBS (fetal bovine serum) (PAA Lab. GmbH, Les Mureaux, France), penicillin/ streptomycin at 100 units/ml and 2 mM L-glutamine as adherent monolayers.

Techniques: Incubation, Control